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MicroResp™ is a unique microplate-based respiration system that enables the user to analyse up to 96 soil, sediment or water samples and test a range of carbon sources and/or replicates in a small compact space.

While some sole C-source tests tend to select for fast-growing bacteria and rely on growth of organisms, MicroResp™ gives more immediate responses to these substrates and reflects activity rather than growth by measuring responses in the first 6 hours.

MicroResp™ is currently being used to carry out colorimetric detection or ¹⁴C detection measuring CO₂ evolved from carbon substrate utilisation in soils. This methodology can be adapted to suit any experimental schedule and microplate reader although calibration is required.

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1. 96-well 1.2ml Deepwell plate - carrier for the carbon source and the test material.
2. 96-well microplate - contains the detection gel or in the case of ¹⁴C detection a 96-well isoplate containing NaOH soaked filter paper.
3. MicroResp™ Seal - a two way rubber sealing mat which connects the two plates together.

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1. Clamp - holds the three main components together during incubation ensuring a good seal between the individual wells, and
2. Filling Device - aids the user in the filling of the deepwell plate with solid material (e.g soil).

Although MicroResp™ was developed to measure CO₂ evolution over short periods (4-6 hours) at 25°C it can be adapted for any time period depending on the sample activity, and for incubation at any temperature, i.e. to mimic natural environmental conditions.

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• Soil health
• Toxicity testing
• Pesticide degradation profiles
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• Microbe strain phenotyping
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